Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1981-7-9
|
| pubmed:abstractText |
A solid phase radioimmunoassay has been developed for the determination of poliovirus D-antigen over the range 1-100 D-units/ml. A dilution series of a D-antigen standard or of the samples to be assayed is incubated in the wells of a flexible vinyl Microtiter plate coated with D-antigen specific antibody. The poliovirus D-antigen bound to the antibody coated wells is quantitated by addition of the same antibody radioactively labelled with n-succinimidyl (2, 3-3H) propionate; the D-antigen content of the samples is determined by means of the standard curve generated by the D-antigen standard dilution series. Both the coating antibody and the radioactively labelled antibody may be freeze dried, and this provides a highly stable assay system with a long shelf-life.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0301-5149
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
47
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
87-90
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1981
|
| pubmed:articleTitle |
A solid phase radioimmunoassay for poliovirus D-antigen.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|