Linked Life Data

6203812

Source:http://linkedlifedata.com/resource/pubmed/id/6203812

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-7-27
pubmed:abstractText
We report here the use of a novel genetic approach for the study of transcriptional control in Streptomyces lividans. Using up-promoter mutants of the ampC beta-lactamase gene of Escherichia coli, we have shown that mutations in each of the regions that define the major determinants of promoter strength in E. coli-i.e., the -35 region, the -10 region, and the intervening "spacer" region-lead to increased synthesis of ampC mRNA in S. lividans, just as they do in their host of origin. Results are also presented showing that the ampC transcriptional terminator is functional in S. lividans. Taken together, these findings indicate that Streptomyces have an RNA polymerase that recognizes and uses the various components of E. coli transcriptional signals, and imply that, notwithstanding the high GC content (i.e., 73%) of the Streptomyces genome, these organisms have indigenous promoters similar to those found in E. coli.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
83-91
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Streptomyces lividans RNA polymerase recognizes and uses Escherichia coli transcriptional signals.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.