Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1983-6-10
pubmed:abstractText
Thymidylate synthase (5,10-methylenetetrahydrofolate:dUMP C-methyltransferase, EC 2.1.1.45) from methotrexate-resistant Streptococcus faecium has a UV absorbance peak at 259 nm and stains with acridine orange because of the presence of RNA on the protein. Material having an absorbance peak at 254 nm, obtained from the enzyme by phenol extraction, is degraded by treatment with pancreatic RNase, T1 RNase, and alkali but is stable to DNase. Dowex-1 chromatography of the pure enzyme yields two polynucleotide fragments in addition to the apoenzyme. As estimated from their absorbance, these fragments contain 4 and 11 mononucleotide residues per mole of enzyme, respectively. In crude extracts, thymidylate synthase is associated with rapidly sedimenting material that is sensitive to RNase. Treatment of crude extracts with RNase, as is done routinely during thymidylate synthase purification, most likely results in the formation of the small polynucleotides found on the enzyme. The RNA is not required for enzyme activity.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-105003, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-108277, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-1247599, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-13767412, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-13895219, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-13998281, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-14240539, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-145246, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-355798, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-4158310, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-4358948, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-4515032, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-4598071, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-4854922, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-4918842, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-4956661, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-5003635, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-5021603, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-5806584, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-6164392, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-7043458, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-714878, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-7391086, http://linkedlifedata.com/resource/pubmed/commentcorrection/6189121-7471094
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
916-20
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Association of RNA with thymidylate synthase from methotrexate-resistant Streptococcus faecium.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.