| pubmed-article:6175357 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6175357 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:6175357 | lifeskim:mentions | umls-concept:C1325559 | lld:lifeskim |
| pubmed-article:6175357 | lifeskim:mentions | umls-concept:C0035681 | lld:lifeskim |
| pubmed-article:6175357 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:6175357 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
| pubmed-article:6175357 | lifeskim:mentions | umls-concept:C0205254 | lld:lifeskim |
| pubmed-article:6175357 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:6175357 | pubmed:dateCreated | 1982-6-21 | lld:pubmed |
| pubmed-article:6175357 | pubmed:abstractText | Rat-liver nucleoli (10-15 micrograms DNA) were digested with either 0.6 or 3 units of DNase I for various times (up to 1 h). RNA synthesis was then measured in the absence or presence of 3 units of Escherichia coli RNA polymerase. It was found that the nucleolar chromatin supporting the endogenous engaged RNA polymerase I transcription was completely destroyed in 3 min with either concentration of DNase I. The nucleolar chromatin template transcribed by E. coli RNA polymerase retained 50% of its original capacity even 60 min after 3 units of DNase I digestion. When hybridization experiments were conducted, it was found that the DNAs derived from both levels of DNase-I-digested nucleoli were incapable of forming hybrids with the labelled nucleolar RNA synthesized by the engaged RNA polymerase I from the untreated nucleoli. Since the engaged RNA polymerase I transcribes only the physiologically active genes of the nucleolar chromatin, and the RNA transcripts represent active gene product, these data suggest that DNase I digestion has completely destroyed the active genes of the nucleolar chromatin, and E. coli RNA polymerase is able to transcribe the inactive nucleolar chromatin template. | lld:pubmed |
| pubmed-article:6175357 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6175357 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6175357 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6175357 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6175357 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6175357 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6175357 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6175357 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6175357 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6175357 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6175357 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6175357 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6175357 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6175357 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:6175357 | pubmed:issn | 0144-8463 | lld:pubmed |
| pubmed-article:6175357 | pubmed:author | pubmed-author:BarretoFF | lld:pubmed |
| pubmed-article:6175357 | pubmed:author | pubmed-author:YuF LFL | lld:pubmed |
| pubmed-article:6175357 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6175357 | pubmed:volume | 2 | lld:pubmed |
| pubmed-article:6175357 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6175357 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6175357 | pubmed:pagination | 155-61 | lld:pubmed |
| pubmed-article:6175357 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:6175357 | pubmed:meshHeading | pubmed-meshheading:6175357-... | lld:pubmed |
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| pubmed-article:6175357 | pubmed:meshHeading | pubmed-meshheading:6175357-... | lld:pubmed |
| pubmed-article:6175357 | pubmed:meshHeading | pubmed-meshheading:6175357-... | lld:pubmed |
| pubmed-article:6175357 | pubmed:meshHeading | pubmed-meshheading:6175357-... | lld:pubmed |
| pubmed-article:6175357 | pubmed:meshHeading | pubmed-meshheading:6175357-... | lld:pubmed |
| pubmed-article:6175357 | pubmed:meshHeading | pubmed-meshheading:6175357-... | lld:pubmed |
| pubmed-article:6175357 | pubmed:meshHeading | pubmed-meshheading:6175357-... | lld:pubmed |
| pubmed-article:6175357 | pubmed:year | 1982 | lld:pubmed |
| pubmed-article:6175357 | pubmed:articleTitle | Evidence for the transcription os physiologically inactive rat-liver nucleolar chromatin by Escherichia coli RNA polymerase. | lld:pubmed |
| pubmed-article:6175357 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6175357 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
