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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1982-6-21
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pubmed:abstractText |
Rat-liver nucleoli (10-15 micrograms DNA) were digested with either 0.6 or 3 units of DNase I for various times (up to 1 h). RNA synthesis was then measured in the absence or presence of 3 units of Escherichia coli RNA polymerase. It was found that the nucleolar chromatin supporting the endogenous engaged RNA polymerase I transcription was completely destroyed in 3 min with either concentration of DNase I. The nucleolar chromatin template transcribed by E. coli RNA polymerase retained 50% of its original capacity even 60 min after 3 units of DNase I digestion. When hybridization experiments were conducted, it was found that the DNAs derived from both levels of DNase-I-digested nucleoli were incapable of forming hybrids with the labelled nucleolar RNA synthesized by the engaged RNA polymerase I from the untreated nucleoli. Since the engaged RNA polymerase I transcribes only the physiologically active genes of the nucleolar chromatin, and the RNA transcripts represent active gene product, these data suggest that DNase I digestion has completely destroyed the active genes of the nucleolar chromatin, and E. coli RNA polymerase is able to transcribe the inactive nucleolar chromatin template.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0144-8463
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
155-61
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:6175357-Animals,
pubmed-meshheading:6175357-Cell Nucleolus,
pubmed-meshheading:6175357-Chromatin,
pubmed-meshheading:6175357-DNA,
pubmed-meshheading:6175357-DNA-Directed RNA Polymerases,
pubmed-meshheading:6175357-Deoxyribonuclease I,
pubmed-meshheading:6175357-Deoxyribonucleases,
pubmed-meshheading:6175357-Endonucleases,
pubmed-meshheading:6175357-Escherichia coli,
pubmed-meshheading:6175357-Liver,
pubmed-meshheading:6175357-Male,
pubmed-meshheading:6175357-Nucleic Acid Hybridization,
pubmed-meshheading:6175357-RNA,
pubmed-meshheading:6175357-RNA Polymerase I,
pubmed-meshheading:6175357-Rats,
pubmed-meshheading:6175357-Rats, Inbred Strains,
pubmed-meshheading:6175357-Templates, Genetic,
pubmed-meshheading:6175357-Transcription, Genetic
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pubmed:year |
1982
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pubmed:articleTitle |
Evidence for the transcription os physiologically inactive rat-liver nucleolar chromatin by Escherichia coli RNA polymerase.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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