Linked Life Data

6174353

Source:http://linkedlifedata.com/resource/pubmed/id/6174353

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
1982-5-21
pubmed:abstractText
Binding studies were performed on rat pancreatic dispersed acini with (--)-[3H]QNB, in parallel with secretory studies to establish that the muscarinic receptors characterized are involved in enzyme secretion. Scatchard analysis of [3H]QNB saturation isotherm gave an apparent KD value of 3.09 X 10(-10) M with binding capacities of 2605 fmol/mg of DNA. The true KD calculated from the ratio of the kinetic parameters was 64 pM. The receptor population can be divided into two classes for the agonist binding sites: a population of high affinity sites and one of low affinity sites. With carbamylcholine, maximal stimulation of amylase release occurred with 40% receptor occupation while occupation of the remaining 60% caused a progressive decrease in stimulation of amylase release. Moreover, the dissociation constant of the high affinity sites for carbamylcholine (1.78 X 10(-6) M) is very close to the ED50 of carbamylcholine (5.81 X 10(-7) M) found for the stimulation of enzyme secretion. These data suggest that the physiological response of the pancreatic acini to a muscarinic agonist would involve the high affinity site population.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0014-2999
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
247-54
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Muscarinic receptor of rat pancreatic acini: [3H]QNB binding and amylase.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't