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rdf:type |
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lifeskim:mentions |
umls-concept:C0002198,
umls-concept:C0004561,
umls-concept:C0024501,
umls-concept:C0034789,
umls-concept:C0039194,
umls-concept:C0243127,
umls-concept:C0332281,
umls-concept:C0443331,
umls-concept:C0721534,
umls-concept:C1521761,
umls-concept:C1948023,
umls-concept:C1999230
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pubmed:issue |
4
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pubmed:dateCreated |
1982-3-26
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pubmed:abstractText |
The supernatant of 'crowded' but not 'spread' rabbit spleen cell cultures contains a macroglobulin factor which behaves in an Ig-turnover assay as any T-independent antigen or polyclonal B-cell activator (PBA). In the supernatants of crowded rabbit lymphoid cell cultures prepared in serum free medium, the factor was found to be associated entirely with the alpha-macroglobulin (alpha M) fraction (alpha 1 + alpha 2). This alpha M was most probably actively secreted by the lymphocytes because: (i) sequential supernatants obtained in serum free medium of crowded cultures contained equal amounts of alpha M as well as equal PBA activity; (ii) the alpha M became labelled when the cells were grown in medium containing a radioactive amino acid. Macrophages were not required for the production of PBA. PBA was not produced when either crowded B or T cells were cultured alone but only when they were cultured together. Purified T cells were not triggered by any plant lectin to produce PBA. By use of anti-alpha 2M allotype antibodies and B and T cells from different rabbits, the PBA was shown to have the allotype of the T-cell donor. The PBA was associated with rabbit alpha 2M but not alpha 1M. We concluded that upon close contact, B cells stimulate T cells to produce a PBA associated with alpha 2M.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-1083869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-1184968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-131174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-152331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-302211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-313259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-313896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-381168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-4097143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-4132599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-4143252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-4187522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-4277646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-4429715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-450142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-4596183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-5411979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-6444546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-6968642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-7365256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-825573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172373-88449
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
44
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
745-54
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6172373-Animals,
pubmed-meshheading:6172373-B-Lymphocytes,
pubmed-meshheading:6172373-Cells, Cultured,
pubmed-meshheading:6172373-Interleukin-1,
pubmed-meshheading:6172373-Proteins,
pubmed-meshheading:6172373-Rabbits,
pubmed-meshheading:6172373-Radioimmunoassay,
pubmed-meshheading:6172373-Receptors, Antigen, B-Cell,
pubmed-meshheading:6172373-Rosette Formation,
pubmed-meshheading:6172373-T-Lymphocytes,
pubmed-meshheading:6172373-alpha-Macroglobulins
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pubmed:year |
1981
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pubmed:articleTitle |
An alpha2-macroglobulin associated factor produced by T lymphocytes which provides polyclonal stimulation of B lymphocytes to maintain the turnover of their surface Ig.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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