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rdf:type |
|
|
lifeskim:mentions |
|
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pubmed:issue |
12
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|
pubmed:dateCreated |
1985-5-16
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|
pubmed:abstractText |
The erythroleukemia cell line K562 was previously shown to have specific binding sites for insulin but not for insulin-like growth factor I (IGF-I). In this study the presence of specific receptors for insulin-like growth factor II (IGF-II) is established. Scatchard analysis of the competition curve for IGF-II disclosed a non-cooperative binding kinetic with a calculated affinity constant 2.4 X 10(8) M-1 and a receptor number of 4.8 X 10(4) sites/cell. IGF-I displayed 10% crossreactivity over the IGF-II receptor but insulin did not crossreact at all. Instead insulin, present in high concentrations, enhanced the binding of IGF-II. The presence of IGF II but not IGF-I receptors makes the K562 cell line suitable for studying properties of the type-2 receptor.
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pubmed:language |
eng
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pubmed:journal |
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|
pubmed:citationSubset |
IM
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|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0144-8463
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1071-7
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:6099156-Binding, Competitive,
pubmed-meshheading:6099156-Binding Sites,
pubmed-meshheading:6099156-Cell Line,
pubmed-meshheading:6099156-Humans,
pubmed-meshheading:6099156-Insulin,
pubmed-meshheading:6099156-Kinetics,
pubmed-meshheading:6099156-Leukemia, Erythroblastic, Acute,
pubmed-meshheading:6099156-Peptides,
pubmed-meshheading:6099156-Receptors, Cell Surface,
pubmed-meshheading:6099156-Receptors, Somatomedin,
pubmed-meshheading:6099156-Somatomedins
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pubmed:year |
1984
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pubmed:articleTitle |
The binding of insulin-like growth factor II to the erythroleukemia cell line K562.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
