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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1972-1-8
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pubmed:abstractText |
1. DNA-dependent RNA polymerase was purified 150-fold from crude extracts of the extreme halophile Halobacterium cutirubrum. 2. The enzyme requires the presence of native DNA and all four nucleoside triphosphates to incorporate (14)C-labelled nucleoside triphosphate into an acid-insoluble ribonuclease-sensitive product. 3. It has an absolute requirement for both Mn(2+) and Mg(2+). 4. The polymerase requires a high salt concentration for stability, but is markedly inhibited by univalent cations. 5. Its molecular weight is very low compared with that of Escherichia coli RNA polymerase.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4940048-13802337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4940048-14114839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4940048-14114841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4940048-14200382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4940048-14450184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4940048-4293779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4940048-4304301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4940048-4860703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4940048-4882047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4940048-4882861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4940048-4900511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4940048-4901708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4940048-4910267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4940048-5114973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4940048-5114974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4940048-5114975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4940048-5227680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4940048-5805399
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Isotopes,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Magnesium,
http://linkedlifedata.com/resource/pubmed/chemical/Manganese,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Nucleotidyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
121
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
621-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:4940048-Carbon Isotopes,
pubmed-meshheading:4940048-DNA,
pubmed-meshheading:4940048-Dialysis,
pubmed-meshheading:4940048-Escherichia coli,
pubmed-meshheading:4940048-Halobacterium,
pubmed-meshheading:4940048-Magnesium,
pubmed-meshheading:4940048-Manganese,
pubmed-meshheading:4940048-Molecular Weight,
pubmed-meshheading:4940048-Phosphates,
pubmed-meshheading:4940048-Proteins,
pubmed-meshheading:4940048-RNA, Bacterial,
pubmed-meshheading:4940048-RNA Nucleotidyltransferases,
pubmed-meshheading:4940048-Radiometry,
pubmed-meshheading:4940048-Sodium Chloride,
pubmed-meshheading:4940048-Spectrophotometry,
pubmed-meshheading:4940048-Tritium,
pubmed-meshheading:4940048-Vibration
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pubmed:year |
1971
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pubmed:articleTitle |
Nucleic acid enzymology of extremely halophilic bacteria. Halobacterium cutirubrum deoxyribonucleic acid-dependent ribonucleic acid polymerase.
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pubmed:publicationType |
Journal Article
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