Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1971-11-16
pubmed:abstractText
The infectivity of intact poliovirus was not affected by exposure to the antibiotic phleomycin at concentrations as high as 200 mug/ml, whereas that of the singlestranded poliovirus ribonucleic acid (RNA) was inactivated to 99% by pretreatment of the RNA with phleomycin at a concentration of 2 mug/ml. The infectivity of double and multistranded RNA was 10 times less sensitive than that of singlestranded RNA to the action of this antibiotic. Preincubation of HeLa cells for 30 min with 10 to 50 mug of phleomycin reduced the sensitivity of the cells to infection by viral RNA and intact virus, indicating that phleomycin interferes with cellular functions necessary for virus replication. When phleomycin was added to cells at different times after infection with single- or double-stranded RNA, the highest inactivation of infective centers was observed immediately after infection. With time of incubation at 37 C, the infective centers became more resistant to the action of phleomycin.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/4328413-13984808, http://linkedlifedata.com/resource/pubmed/commentcorrection/4328413-13997383, http://linkedlifedata.com/resource/pubmed/commentcorrection/4328413-14134888, http://linkedlifedata.com/resource/pubmed/commentcorrection/4328413-14292780, http://linkedlifedata.com/resource/pubmed/commentcorrection/4328413-14309404, http://linkedlifedata.com/resource/pubmed/commentcorrection/4328413-14410319, http://linkedlifedata.com/resource/pubmed/commentcorrection/4328413-4289760, http://linkedlifedata.com/resource/pubmed/commentcorrection/4328413-4290003, http://linkedlifedata.com/resource/pubmed/commentcorrection/4328413-4290827, http://linkedlifedata.com/resource/pubmed/commentcorrection/4328413-4297402, http://linkedlifedata.com/resource/pubmed/commentcorrection/4328413-4305631, http://linkedlifedata.com/resource/pubmed/commentcorrection/4328413-4314502
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
28-34
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1971
pubmed:articleTitle
Differential effect of phleomycin on the infectivity of poliovirus and poliovirus-induced ribonucleic acids.
pubmed:publicationType
Journal Article