Linked Life Data

4053563

Source:http://linkedlifedata.com/resource/pubmed/id/4053563

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1985-12-19
pubmed:abstractText
A method for estimating type II to type I collagen ratios in small tissue samples has been developed. The cyanogen bromide peptides of the tissue collagens were analyzed by SDS-gel electrophoresis. Marker peptides representative of each collagen type were established and their relative amounts determined by integration of the stained peptide bands following gel scans. Marker peptide ratios were then computed for each of several standard type II/type I mixtures and these peptide ratios were mathematically correlated with the corresponding type II/type I collagen ratios. A linear relationship between marker peptide ratio and collagen type ratio was established. This relationship was applied to the analyses of type II/type I ratios in samples of rib perichondrium and neocartilage derived from perichondrial graft repairs of full thickness femoral condyle defects. The results indicated that perichondrial grafts synthesize both types II and I collagens and that the proportion of type II increases with increasing post-transplant time.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0174-173X
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
337-47
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Collagen types in neocartilage tissue resulting from rib perichondrial graft in an articular defect--a rapid semi-quantitative methodology.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't