Linked Life Data

3967784

Source:http://linkedlifedata.com/resource/pubmed/id/3967784

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1985-3-20
pubmed:abstractText
Removal of cumulus may increase the chance of fertilization in patients with sperm antibodies, may facilitate fertilization in vitro with a small number of spermatozoa, and is necessary for microsurgical injection procedures in vitro. The aim of this study was to establish whether removal of the cumulus has any detrimental effects on the fertilization rate and embryo viability. Removal of cumulus cells from the human oocyte with bovine testicular hyaluronidase did not interfere with fertilization, early embryonic development, or pregnancy. This suggests that human spermatozoa can spontaneously undergo capacitation and fertilize oocytes in vitro in a chemically defined medium containing 10% preovulatory human serum. In three patients with low-quality semen, removal of the cumulus and the addition of hypotaurine and epinephrine apparently did not improve the motility nor the fertilizing capacity of the spermatozoa. Delayed fertilization and cleavage arrest was observed in one patient when spermatozoa obtained from the body region of the epididymis was used for insemination.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0015-0282
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
263-7
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Removal of the cumulus oophorus from the human oocyte for in vitro fertilization.
pubmed:publicationType
Journal Article