Linked Life Data

3936537

Source:http://linkedlifedata.com/resource/pubmed/id/3936537

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
1986-2-28
pubmed:abstractText
In Drosophila, the vast majority of mRNAs that are polysome associated during oogenesis are also polysome associated during early embryogenesis. We have previously identified an exceptional mRNA that appears to be depleted from early-embryo polysomes [Fruscoloni, P., Al-Atia, G. R., & Jacobs-Lorena, M. (1983) Proc. Natl. Acad. Sci. U.S.A. 80, 3359-3363]. This mRNA has been subsequently identified as coding for a ribosomal protein (r-protein) [Kay, M., & Jacobs-Lorena, M. (1985) Mol. Cell. Biol. (in press)]. Changes in association with polysomes of two r-protein mRNAs during early Drosophila development were investigated for this report. Hybridization of cloned DNA probes to blots of RNA obtained from sucrose gradient fractions reveals that r-protein mRNAs are substantially associated with polysomes during oogenesis, depleted from polysomes during early embryogenesis, and again polysome associated during late embryogenesis. Thus, translation of r-protein mRNAs parallels transcription of ribosomal RNA (rRNA) during this time of development. By contrast, no such differences were observed when actin and histone probes were used as controls and hybridized to the same blots. The abundance of mRNAs for r-proteins as a function of development was also measured. Abundance was relatively high and constant during oogenesis and embryogenesis (when translational regulation is apparent), somewhat decreased in larval and pupal stages, and low in adult nonovarian tissues. Coordination between r-protein and rRNA synthesis appears to be achieved by regulating translation of r-protein mRNAs in early embryos and by decreasing their abundance in adult tissues.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5798-803
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Translational regulation of mRNAs for ribosomal proteins during early Drosophila development.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.