Linked Life Data

3733684

Source:http://linkedlifedata.com/resource/pubmed/id/3733684

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
1986-9-17
pubmed:abstractText
Human fibroblasts were insensitive to tumor necrosis factor (TNF) alone but were killed when treated with TNF together with the inhibitor of protein synthesis cycloheximide. However, incubation with TNF before addition of cycloheximide protected these cells from cytotoxicity. This suggested that TNF induced some protective mechanism(s) and that protein synthesis was required for this induction. By labeling fibroblasts with [35S]methionine, we observed that TNF induced in these cells the synthesis of two proteins of Mr 36,000 and 42,000 (p36 and p42). This induction was dependent on the TNF concentration and was observed within a few hours after addition of TNF. Actinomycin D prevented synthesis of these proteins, suggesting that TNF induced transcription of the corresponding mRNAs. p42 appeared to be stable, whereas p36 turned over within a few hours. The physiological role of these proteins in the cellular response to TNF remains to be established.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
261
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9565-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Tumor necrosis factor induces synthesis of two proteins in human fibroblasts.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.