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Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1986-6-12
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pubmed:abstractText |
Chicken oviduct progesterone receptor in cytosol was found to be transformed from the 8S to 4S form by incubation at 25 degrees C as well as by 0.3 M KCl in the absence of hormone. Heat transformation of ligand-free receptor took place at a much slower rate than that of ligand-bound receptor. The eventual percentage of transformation, however, was almost the same. The 4S form of the receptor transformed by KCl in the absence of hormone could bind to DNA-cellulose, but not to nuclei. However, upon exposure it acquired the ability to bind to nuclei. It was shown that the transformed ligand-free receptor could bind to progesterone to form the normal activated steroid-receptor complex. Conversely, when activated 4S progesterone-receptor complex was treated with DCC to peel off the hormone, a resulting ligand-free receptor was formed which behaved just like the KCl-transformed receptor in the absence of hormone.
|
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
0022-4731
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
24
|
pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
|
pubmed:pagination |
215-8
|
pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:3702405-Animals,
pubmed-meshheading:3702405-Chickens,
pubmed-meshheading:3702405-Chromatography, DEAE-Cellulose,
pubmed-meshheading:3702405-Female,
pubmed-meshheading:3702405-Hot Temperature,
pubmed-meshheading:3702405-Ligands,
pubmed-meshheading:3702405-Oviducts,
pubmed-meshheading:3702405-Potassium Chloride,
pubmed-meshheading:3702405-Progesterone,
pubmed-meshheading:3702405-Receptors, Progesterone
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pubmed:year |
1986
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pubmed:articleTitle |
Transformation of the chick oviduct progesterone receptor in the absence of hormone.
|
pubmed:publicationType |
Journal Article,
In Vitro
|