Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0003241,
umls-concept:C0010453,
umls-concept:C0016701,
umls-concept:C0020204,
umls-concept:C0021027,
umls-concept:C0025663,
umls-concept:C0040300,
umls-concept:C0086418,
umls-concept:C0205155,
umls-concept:C0205227,
umls-concept:C0220908,
umls-concept:C0332206,
umls-concept:C1522472,
umls-concept:C1548793,
umls-concept:C1550101,
umls-concept:C1551341,
umls-concept:C1552858,
umls-concept:C1552923,
umls-concept:C1552924,
umls-concept:C1705191
|
| pubmed:issue |
1
|
| pubmed:dateCreated |
1987-12-30
|
| pubmed:abstractText |
Endogenous immunoglobulin in tissue sections pose a problem in immuno-histochemical techniques employing homologous antibody as primary reagents and enzyme-labelled anti-immunoglobulin for the development. A method for the blocking of endogenous immunoglobulin in human tissue sections by incubation with monomeric pepsin fragments (Fab') of rabbit anti-human immunoglobulin before applying monoclonal antibody was evaluated for the screening of human monoclonal antibody. It was initially demonstrated that Fab' rabbit anti-human IgM and anti-IgG could block endogenous immunoglobulin in human IgM and IgG producing tumors thereby abolishing the binding of subsequently applied peroxidase-labelled anti-IgM or anti-IgG. Frozen sections of human colo-rectal adenocarcinomas show a variable background staining caused by the endogenous immunoglobulin. The background completely disappeared in the IgM system by preincubation with Fab' anti-IgM while the background was clearly reduced but not abolished in the IgG system. A human hybridoma supernatant containing IgM reactive with colo-rectal adenocarcinoma could easily be screened on frozen sections using this method. This approach should be generally useful for the screening of human antibody on human tissue sections.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0272-457X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
6
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
103-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:3679252-Antibodies, Monoclonal,
pubmed-meshheading:3679252-Colonic Neoplasms,
pubmed-meshheading:3679252-Humans,
pubmed-meshheading:3679252-Hybridomas,
pubmed-meshheading:3679252-Immunoglobulin G,
pubmed-meshheading:3679252-Immunoglobulin M,
pubmed-meshheading:3679252-Immunoglobulins,
pubmed-meshheading:3679252-Lymphoma,
pubmed-meshheading:3679252-Plasmacytoma,
pubmed-meshheading:3679252-Rectal Neoplasms
|
| pubmed:year |
1987
|
| pubmed:articleTitle |
A method for the blocking of endogenous immunoglobulin on frozen tissue sections in the screening of human hybridoma antibody in culture supernatants.
|
| pubmed:affiliation |
Institute of Pathology, University of Odense, Denmark.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|