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rdf:type |
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lifeskim:mentions |
umls-concept:C0000661,
umls-concept:C0001657,
umls-concept:C0008381,
umls-concept:C0010453,
umls-concept:C0018207,
umls-concept:C0033414,
umls-concept:C0035696,
umls-concept:C0086418,
umls-concept:C0205178,
umls-concept:C0441712,
umls-concept:C0442805,
umls-concept:C1141639
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pubmed:issue |
3
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pubmed:dateCreated |
1987-10-7
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pubmed:abstractText |
Treatment of human granulosa cells with human chorionic gonadotropin (hCG) or an analogue of its second messenger, cyclic AMP (cAMP), promotes a rapid accumulation of the messenger RNAs (mRNAs) for cytochrome P450 side-chain cleavage (scc) and adrenodoxin. A twofold increase in the cellular content of these mRNAs was observed within 4 h of exposure to 8-bromo-cAMP, and was maintained for up to 48 h. Inhibition of protein synthesis by cycloheximide did not prevent the hCG- or 8-bromo-cAMP-stimulated accumulation of either cytochrome P450scc or adrenodoxin mRNAs. We conclude that human granulosa cells respond rapidly to hCG and cAMP analogues with a coordinate increase in levels of the mRNAs encoding two key proteins of the steroidogenic machinery, and that this stimulation does not require synthesis of a protein intermediate.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3624492-2419119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3624492-2423394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3624492-2840565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3624492-2984060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3624492-2994043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3624492-3011839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3624492-3014507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3624492-3024157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3624492-3298591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3624492-3794551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3624492-4031008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3624492-6275662
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
896-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3624492-8-Bromo Cyclic Adenosine Monophosphate,
pubmed-meshheading:3624492-Adrenodoxin,
pubmed-meshheading:3624492-Biomechanics,
pubmed-meshheading:3624492-Chorionic Gonadotropin,
pubmed-meshheading:3624492-Cycloheximide,
pubmed-meshheading:3624492-Cytochrome P-450 Enzyme System,
pubmed-meshheading:3624492-Female,
pubmed-meshheading:3624492-Granulosa Cells,
pubmed-meshheading:3624492-Humans,
pubmed-meshheading:3624492-RNA, Messenger
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pubmed:year |
1987
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pubmed:articleTitle |
Human chorionic gonadotropin and 8-bromo cyclic adenosine monophosphate promote an acute increase in cytochrome P450scc and adrenodoxin messenger RNAs in cultured human granulosa cells by a cycloheximide-insensitive mechanism.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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