Linked Life Data

3620112

Source:http://linkedlifedata.com/resource/pubmed/id/3620112

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1987-10-15
pubmed:abstractText
A 500-base cDNA encoding the bovine protamine was used for hybridization experiments with total RNA prepared from testes of prepubertal and sexually mature bulls and from pachytene spermatocytes and spermatids isolated from mature testes. The mRNA for protamine was first detected in the 7-month old testis containing 10-15% of round spermatids but was absent in testes of younger animals containing only diploid spermatogenic cells. Hybridization of the protamine cDNA with the RNA of isolated spermatids of the mature testis resulted in a prominent hybridization signal, while the faint signal obtained with the RNA of pachytene spermatocytes was found to be due to contamination of the cell preparation by spermatids. As demonstrated by in situ hybridization on testis-sections the transcripts are confined to the central cell layers of the tubuli seminiferi corresponding to the spatial arrangement of postmeiotic spermatogenic cells. The results indicate that the protamine gene in the bull is postmeiotically expressed and the mRNA is synthesized as a 680 nucleotide long molecule.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0177-3593
pubmed:author
pubmed:issnType
Print
pubmed:volume
368
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
807-11
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Haploid expression of a protamine gene during bovine spermatogenesis.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't