Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1987-7-27
pubmed:abstractText
Dictyate oocytes were isolated from the ovaries of 22-day-old mice and cultured for 24 h. Isolation and classification of the oocytes were done at a stable pH of 7.45, during gradual increase of pH from 7.45 to 8.3, or at pH 8.3. In cumulus-enclosed oocytes alkalization beyond pH 7.45 resulted in an increased rate of degeneration and decreased rates of germinal vesicle breakdown and polar body formation. In the naked oocytes, only the rate of degeneration was affected. The results stress the importance of a stable pH during the period of isolation, and of a careful classification of the oocytes before culture.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-4251
pubmed:author
pubmed:issnType
Print
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
251-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Maturation of mouse oocytes in vitro is influenced by alkalization during their isolation.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't