Linked Life Data

3549193

Source:http://linkedlifedata.com/resource/pubmed/id/3549193

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1987-5-11
pubmed:abstractText
Alterations in the organization of the microtubular cytoskeleton and chromosome alignment were examined by tubulin immunofluorescence and DAPI staining during in vivo ageing of naturally ovulated, metaphase-arrested oocytes of CBA/Ca mice in the fallopian tubes. In oocytes isolated from young mice on the day of oestrus, a few hours after ovulation, when they are still tightly surrounded by cumulus, the anti-tubulin fluorescence is almost exclusively restricted to the metaphase spindle. Only some faintly staining foci are observed in the cytoplasm, which presumably represent cytoplasmic MTOC not involved in spindle formation. The spindle is usually barrel-shaped or slightly pointed at its poles and does not possess astral fibres. In oocytes aged for more than 12 h in the fallopian tubes cytoplasmic asters develop, while microtubules seem to become gradually lost from the spindle, preferentially in its central area near the chromosomes. Astral fibres are observed radiating out from the polar centrosomes into the cytoplasm. In oocytes free of cumulus, and consequently more than 24 h post-ovulation, a pronounced shrinking of the spindle is observed. The mean pole-to-pole distance becomes significantly reduced in postovulatory aged cells. At the same time astral microtubules in the cytoplasm appear to become gradually depolymerized. Age-dependent alterations in the microtubular cytoskeleton do not seem to result from a changed pattern of the post-translational detyrosylation of alpha-tubulin in certain sets of microtubules. In freshly ovulated oocytes chromosomes in most spindles are well ordered and precisely arranged at the equatorial plane.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0009-5915
pubmed:author
pubmed:issnType
Print
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
337-45
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Alterations to the microtubular cytoskeleton and increased disorder of chromosome alignment in spontaneously ovulated mouse oocytes aged in vivo: an immunofluorescence study.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't