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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1986-9-17
|
| pubmed:abstractText |
The antisecretory factors (ASF) are hormone-like proteins which inhibit cholera toxin-induced intestinal hypersecretion. Although ASF concentrations in young control rats were low, those in old control rats and toxin-treated rats were high. Toxin-treated rats had 200 ED50 units/g wet weight of ASF in the pituitary gland, while their intestinal mucosa, bile and milk contained 3, 0.5 and 0.5 units/g. In adult man and in 8-9-month-old pig the pituitary level was about 20 units/g. The isoelectric points of ASF from pig and rat were 4.8 and 5.0, respectively, while the molecular size as determined by gel filtration on Bio-Gel P-150 was the same in both cases (Kav 0.43). The molecular weight as determined by SDS-polyacrylamide gel electrophoresis was 60,000 for ASF from porcine pituitary gland. One ED50 unit of the purified porcine ASF corresponded to about 10(-13) mol (1-5 ng) of protein. There were two different ASF from human pituitary gland: pI 5.2, Kav 0.43; and pI 4.5, Kav 0.6. Since antibodies against porcine ASF failed to neutralize the latter protein, it may be unrelated to porcine ASF; the human pI 5.2-protein and rat ASF were both neutralized, but less effectively than was porcine ASF. All the ASF molecules attached to agarose gel, from which they dissociated again in methyl alpha-D-glucose: porcine and rat ASF were eluted at 0.3-0.9 M methyl alpha-D-glucose, human pI 5.2-ASF at 0.1-0.9 M, and human pI 4.5-ASF at 0.1-1.5 M methyl alpha-D-glucose.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0006-3002
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
6
|
| pubmed:volume |
883
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
138-44
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:3524692-Adult,
pubmed-meshheading:3524692-Aged,
pubmed-meshheading:3524692-Aging,
pubmed-meshheading:3524692-Animals,
pubmed-meshheading:3524692-Bile,
pubmed-meshheading:3524692-Cholera Toxin,
pubmed-meshheading:3524692-Chromatography, Affinity,
pubmed-meshheading:3524692-Chromatography, Gel,
pubmed-meshheading:3524692-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3524692-Female,
pubmed-meshheading:3524692-Humans,
pubmed-meshheading:3524692-Immunologic Techniques,
pubmed-meshheading:3524692-Intestinal Mucosa,
pubmed-meshheading:3524692-Intestines,
pubmed-meshheading:3524692-Isoelectric Focusing,
pubmed-meshheading:3524692-Male,
pubmed-meshheading:3524692-Middle Aged,
pubmed-meshheading:3524692-Milk,
pubmed-meshheading:3524692-Nerve Tissue Proteins,
pubmed-meshheading:3524692-Neuropeptides,
pubmed-meshheading:3524692-Pituitary Gland,
pubmed-meshheading:3524692-Rats,
pubmed-meshheading:3524692-Rats, Inbred Strains,
pubmed-meshheading:3524692-Swine
|
| pubmed:year |
1986
|
| pubmed:articleTitle |
Purification and characterization of the antisecretory factor: a protein in the central nervous system and in the gut which inhibits intestinal hypersecretion induced by cholera toxin.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|