Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1986-7-9
pubmed:abstractText
Mouse tumor necrosis factor (TNF) was purified from serum through a series of steps, and each step was monitored for L-cell cytotoxicity in vitro and tumor-necrotizing activity in vivo. The two activities copurified and could not be dissociated. Purified mouse TNF has a specific activity of 2.2 X 10(7) (L-cell assay in the absence of actinomycin D) and 1 microgram causes necrosis of the standard TNF-sensitive sarcoma Meth A. TNF has a Mr of 39,000 +/- 2000 by gel filtration and a Mr of 16,000-18,000 by NaDodSO4/PAGE. Both molecular weight forms display cytotoxic and necrotizing activities. TNF has a pI of 3.9 and is destroyed by trypsin, protease, elastase, and alpha-chymotrypsin but not by neuraminidase or papain. These characteristics of nonrecombinant mouse TNF clearly resemble those of recombinant human and mouse TNF.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-1000787, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-1103152, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-1207755, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-2989794, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-2993471, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-333124, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-3856324, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-3863119, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-3871770, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-3883195, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-3898078, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-3932069, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-4030139, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-54919, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-6177893, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-6193516, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-6334807, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-6386027, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-6391657, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-6392892, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-6469400, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-6528335, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-6595667, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-6608523, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-698047, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-7002614, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-7002801, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-7004632, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-7204965, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-7300034, http://linkedlifedata.com/resource/pubmed/commentcorrection/3520561-7339069
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
83
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3949-53
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Purification, characterization, and antitumor activity of nonrecombinant mouse tumor necrosis factor.
pubmed:publicationType
Journal Article