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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1987-9-21
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pubmed:abstractText |
Progesterone receptors were immunoprecipitated with monoclonal antibodies KD68 from lysates of human breast carcinoma T47D cells labelled to steady state specific activity with 32Pi. The 120 kDa 32P-labelled progesterone receptor band was resolved by polyacrylamide gel electrophoresis and identified by autoradiography. Phosphoamino acid analysis revealed serine phosphorylation, but no threonine or tyrosine phosphorylation. Treatment of the 32Pi-labelled cells with EGF, TPA or dibutyryl cAMP had no significant quantitative effect on progesterone receptor phosphorylation, though the EGF receptor and the cAMP-dependent protein kinases have been reported to catalyze phosphorylation of purified avian progesterone receptor preparations in cell free systems. Progesterone receptor phosphorylation on serine residues was increased by 2-fold in cells treated with 10 nM progesterone; EGF had no effect on progesterone-mediated progesterone receptor phosphorylation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
146
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1357-65
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:3497636-Antibodies, Monoclonal,
pubmed-meshheading:3497636-Breast Neoplasms,
pubmed-meshheading:3497636-Cell Line,
pubmed-meshheading:3497636-Enzyme Activation,
pubmed-meshheading:3497636-Epidermal Growth Factor,
pubmed-meshheading:3497636-Female,
pubmed-meshheading:3497636-Humans,
pubmed-meshheading:3497636-Kinetics,
pubmed-meshheading:3497636-Ligands,
pubmed-meshheading:3497636-Phosphates,
pubmed-meshheading:3497636-Phosphorus Radioisotopes,
pubmed-meshheading:3497636-Phosphorylation,
pubmed-meshheading:3497636-Progesterone,
pubmed-meshheading:3497636-Protein Kinases,
pubmed-meshheading:3497636-Receptors, Progesterone
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pubmed:year |
1987
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pubmed:articleTitle |
Cellular progesterone receptor phosphorylation in response to ligands activating protein kinases.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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