Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1987-7-8
pubmed:abstractText
A monoclonal anti-idiotypic (anti-Id) antibody, HF1.7, was generated against anti-Leu-3a, a mouse monoclonal antibody (mAb) specific for the CD4 molecule on human helper/inducer T lymphocytes. The anti-Id nature of HF1.7 was demonstrated by the following properties. (i) It reacted in a solid-phase immunoassay with anti-Leu-3a and not with a panel of irrelevant mouse mAbs. (ii) It partially inhibited the binding of anti-Leu-3a to CD4+ T cells. (iii) It detected a common idiotype present on various anti-CD4 mAbs. Because the CD4 molecule represents the receptor site for human immunodeficiency virus (HIV), the etiologic viral agent of acquired immunodeficiency syndrome, we examined the ability of the anti-mAb HF1.7 to mimic CD4 and bind HIV. This anti-Id mAb reacted with HIV antigens in commercial HIV ELISAs and recognized HIV-infected human T cells but not uninfected cells when analyzed by flow cytofluorometry. Attesting further to the HIV specificity, the anti-Id mAb reacted with a recombinant gp160 peptide and a molecule of Mr 110,000-120,000 in immunoblot analysis of HIV-infected cell lysates. The anti-Id mAb also partially neutralized HIV infection of human T cells in vitro. These results strongly suggest that this anti-Id mAb mimics the CD4 antigenic determinants involved in binding to HIV.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-14097352, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-2409201, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-2423100, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-2428879, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-2430333, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-2989831, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-2995487, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-2997922, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-3001934, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-3006246, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-3011909, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-3014648, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-3014996, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-3023102, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-3157757, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-3466790, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-3487828, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-3856626, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-3871949, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-3952505, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-4142565, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-6083454, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-6096719, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-6313806, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-6328660, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-6815270, http://linkedlifedata.com/resource/pubmed/commentcorrection/3495801-825576
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3891-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Monoclonal anti-idiotypic antibody mimics the CD4 receptor and binds human immunodeficiency virus.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.