Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1987-10-27
pubmed:abstractText
A new, rapid, and sensitive method for assaying phenol sulfotransferase activity toward 2-naphthol is described. The product 2-naphthyl sulfate is quantitated fluorometrically. Optimal wavelengths for excitation and emission were determined by recording the three-dimensional fluorescence spectra of the substrate and the product. The new method is applicable to crude cell or tissue homogenates as well as to further purified preparations. A comparison to another widely used method is given to point out the advantages provided by the new procedure. In particular, sensitivity and accuracy of both methods are evaluated and the influence of interfering substances on both systems is compared. These results clearly indicate the superiority of the new method.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:volume
163
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
546-51
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
A fluorometric assay for quantitating phenol sulfotransferase activities in homogenates of cells and tissues.
pubmed:affiliation
Institute of Toxicology, University of Mainz, Federal Republic of Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't