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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1988-5-3
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pubmed:abstractText |
Tumour necrosis factor (TNF) was previously shown to suppress lipoprotein lipase (LPL) synthesis and activity in 3T3-L1 adipocytes. The present study examined the effect of TNF on amounts of mRNA for LPL in 3T3-L1 cells. Northern-blot analysis of polyadenylated RNA using a cDNA probe to guinea-pig LPL identified two predominant species of LPL message, 3.7 and 3.9 kilobases in size. The steady-state amounts of these mRNAs increased 10-fold upon expression of the adipocyte phenotype. A single dose of 1.5 nM-TNF decreased LPL mRNA by approx. 60% in 17 h with a corresponding decrease in LPL activity, an effect that was reversed 48 h after exposure to TNF. The results demonstrate that TNF reversibly down-regulates LPL mRNA in fully differentiated 3T3-L1 adipocytes. Cells induced to differentiate in the presence of 1.5 nM-TNF exhibited a delayed time course for development of the adipocyte phenotype, as judged by attenuation of the normal increase in LPL mRNA that occurs with differentiation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-166845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-23905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-2430284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-2885834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-2999235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-3010124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-3027100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-3093625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-3491626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-3510667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-3543052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-3597382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-3611087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-3800396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-3814100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-3823907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-3839597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-3872925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-3884609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-3955064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-3988766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-5648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-6066623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-6893015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-6989270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-7038689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-7276825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-7464858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3355496-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
249
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
765-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3355496-Adipose Tissue,
pubmed-meshheading:3355496-Animals,
pubmed-meshheading:3355496-Cell Line,
pubmed-meshheading:3355496-Electrophoresis, Agar Gel,
pubmed-meshheading:3355496-Lipoprotein Lipase,
pubmed-meshheading:3355496-Mice,
pubmed-meshheading:3355496-Molecular Weight,
pubmed-meshheading:3355496-Nucleic Acid Hybridization,
pubmed-meshheading:3355496-RNA, Messenger,
pubmed-meshheading:3355496-Tumor Necrosis Factor-alpha
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pubmed:year |
1988
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pubmed:articleTitle |
Regulation of lipoprotein lipase mRNA content in 3T3-L1 cells by tumour necrosis factor.
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pubmed:affiliation |
Department of Biochemistry, East Carolina University School of Medicine, Greenville, NC 27858.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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