Linked Life Data

3321817

Source:http://linkedlifedata.com/resource/pubmed/id/3321817

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1988-1-25
pubmed:abstractText
Cells from malignant and nonmalignant lesions of the breast were suspended in three different fixatives or in a balanced electrolyte solution (Hank's), stored for varying periods of time, collected on Millipore filters and then imprinted on to clean microslides in order to evaluate the influence of prefixation and fixation time on epithelial cellularity and cellular preservation. The use of a methanol-acetic acid fixative (Esposti's fixative) or 50% isopropanol resulted in good preservation whereas cells prefixed in formaldehyde or 100% isopropanol were poorly preserved. Cells that had not been prefixed (suspended in Hank's solution) showed fair preservation. Eighty-eight percent of the imprints prepared from suspensions of Esposti's fixative were highly cellular, which was significantly better than with Hank's solution (68%), 50% isopropanol (66%), 100% isopropanol (56%) and formaldehyde (33%). The cellularity of the formaldehyde-prefixed imprints differed significantly from the others. There was no influence of storage time on either cellular preservation or epithelial cellularity for any of the investigated solutions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0001-5547
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
950-3
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:articleTitle
Influence of prefixation and fixation times on cellular preservation and epithelial cellularity in filter imprints.
pubmed:affiliation
Department of Pathology, Odense Sygehus, Denmark.
pubmed:publicationType
Journal Article