Linked Life Data

3312252

Source:http://linkedlifedata.com/resource/pubmed/id/3312252

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1987-12-17
pubmed:abstractText
Mitotic spindles were isolated from Chinese hamster ovary (CHO) cells and examined morphologically and biochemically. The isolated spindles were observed to be intact structures containing associated chromosomes and were surrounded by a cage of vimentin-containing filaments. Two-dimensional gel electrophoresis of isolated spindles versus whole cell homogenates indicated that isolated spindles were free from significant cytoplasmic contamination and contained tubulin, actin, vimentin and an 80 X 10(3) Mr quadrapeptide as their major protein constituents. Five calmodulin-binding proteins with molecular weights of 200, 160, 130, 60 and 52 (X 10(3] Mr were identified within isolated spindles. These calmodulin-binding proteins may be involved in regulating microtubule organization and depolymerization during karyokinesis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:volume
87 ( Pt 5)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
643-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Biochemical characterization of isolated CHO cell mitotic spindles: identification of calmodulin-binding proteins.
pubmed:affiliation
Department of Medicine, University of Texas Medical School, Houston 77025.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't