Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1987-11-5
pubmed:abstractText
The biosynthesis of insulin and betagranin, a 20-21 kDa co-secreted chromogranin A-related protein, were investigated in isolated insulinoma cells and islets. The insulinoma tissue processed proinsulin to insulin with kinetics similar to those reported in islet tissue. Unlike islets, however, the insulinoma released almost one-quarter of the newly synthesized proinsulin into the medium 10-40 min after its formation. Betagranin was initially immunoprecipitated as a 100 kDa precursor form, which was indistinguishable from chromogranin A in size and immunoreactivity and by peptide mapping. After an initial lag of 10-20 min, the precursor was converted progressively into betagranin, which appeared to be a stable end product. Formation of betagranin and insulin from their respective precursors followed a parallel course and could likewise be inhibited by NH4+, chloroquine and monensin, added either before labelling or at any point of time up to 15 min after labelling. As with proinsulin, approximately one-quarter of the betagranin precursor was released 10-40 min after synthesis. It is concluded that betagranin is produced by limited proteolysis from a chromogranin A precursor in pancreatic beta-cells by a cellular pathway indistinguishable from that of insulin from proinsulin. Chromogranin A is highly conserved in the N-terminal region represented by betagranin, further suggesting that the biological activity of chromogranin A may reside in a derived peptide rather than in the parent molecule.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-191819, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-3018587, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-359597, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-3755681, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-3881437, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-3896518, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-3896848, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-3943617, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-3969832, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-3990959, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-4351967, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-4370934, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-4962084, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-5333500, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-6117493, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-6126183, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-6202578, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-6207064, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-6209182, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-6272721, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-6300682, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-6311629, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-6322863, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-6391571, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-6759269, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-7041809, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-7421597, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-849482, http://linkedlifedata.com/resource/pubmed/commentcorrection/3311030-942051
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
244
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
449-56
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Biosynthesis of betagranin in pancreatic beta-cells. Identification of a chromogranin A-like precursor and its parallel processing with proinsulin.
pubmed:affiliation
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, U.K.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't