Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1988-5-20
pubmed:abstractText
Monoclonal antibodies raised against purified chicken progesterone receptor (PgR) have been described and characterized recently. In this study we have screened these antibodies for cross-reactivity with murine PgR. Of the six anti-PgR antibodies tested, one (alpha PR6) precipitates murine PgR in an assay using protein A-sepharose as an absorbent for the antibody. The antibody is specific for PgR and does not react with the estrogen receptor or the glucocorticoid receptor in the same cytosol. In immunoblot experiments, both alpha PR6 and alpha PR11 recognize a 115,000 Da protein, however, alpha PR11 gives a weaker signal than alpha PR6. In photoaffinity labeling experiments, a 115,000 Da and an 83,000 Da protein covalently bind tritiated R5020 in a receptor-specific way. We conclude that the alpha PR6 antibody can be used as a tool to study the structure and function of the murine PgR.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-4731
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
297-306
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:3282125-Affinity Labels, pubmed-meshheading:3282125-Animals, pubmed-meshheading:3282125-Antibodies, Monoclonal, pubmed-meshheading:3282125-Antibody Specificity, pubmed-meshheading:3282125-Antigens, pubmed-meshheading:3282125-Centrifugation, Density Gradient, pubmed-meshheading:3282125-Chickens, pubmed-meshheading:3282125-Cytosol, pubmed-meshheading:3282125-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:3282125-Female, pubmed-meshheading:3282125-Immunoenzyme Techniques, pubmed-meshheading:3282125-Immunosorbent Techniques, pubmed-meshheading:3282125-Mice, pubmed-meshheading:3282125-Mice, Inbred BALB C, pubmed-meshheading:3282125-Photochemistry, pubmed-meshheading:3282125-Rats, pubmed-meshheading:3282125-Rats, Inbred Strains, pubmed-meshheading:3282125-Receptors, Progesterone, pubmed-meshheading:3282125-Species Specificity, pubmed-meshheading:3282125-Uterus
pubmed:year
1988
pubmed:articleTitle
Interaction of murine progesterone receptors with specific monoclonal antibodies to the avian progesterone receptor.
pubmed:affiliation
Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, Montreal, Quebec, Canada.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't