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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1988-3-11
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pubmed:abstractText |
The gene for human granulocyte/macrophage colony-stimulating factor (GM-CSF) is expressed in a tissue-specific as well as an activation-dependent manner. The interaction of nuclear proteins with the promoter region of the GM-CSF gene that is likely to be responsible for this pattern of GM-CSF expression was investigated. We show that nuclear proteins interact with DNA fragments from the GM-CSF promoter in a cell-specific manner. A region spanning two cytokine-specific sequences, cytokine 1 (CK-1, 5' GAGATTCCAC 3') and cytokine 2 (CK-2, 5' TCAGGTA 3') bound two nuclear proteins [nuclear factor (NF)-GMa and NF-GMb] from GM-CSF-expressing cells in gel retardation assays. NF-GMb was inducible with phorbol 12-myristate 13-acetate and accompanied induction of GM-CSF message. NF-GMb was absent in cell lines not producing GM-CSF, some of which had other distinct binding proteins. NF-GMa and NF-GMb eluted from a heparin-Sepharose column at 0.3 and 0.6 M KCl, respectively. We hypothesize that the sequences CK-1 and CK-2 bind specific proteins and regulate GM-CSF transcription.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-2423327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-2992804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-2999978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3021817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3025857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3091258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3093895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3095662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3102073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3105621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3296190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3296191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3484428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3484837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3488815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3489188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3490669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3494605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3708688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3708689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3871704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3871951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3876930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3876931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3923623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-3926497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-6240025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-6244013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-6316341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-6600255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-6608729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-6828386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-714156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3257571-7459860
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
674-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3257571-Base Sequence,
pubmed-meshheading:3257571-Cell Line,
pubmed-meshheading:3257571-Colony-Stimulating Factors,
pubmed-meshheading:3257571-DNA,
pubmed-meshheading:3257571-DNA-Binding Proteins,
pubmed-meshheading:3257571-Granulocytes,
pubmed-meshheading:3257571-Humans,
pubmed-meshheading:3257571-Macrophages,
pubmed-meshheading:3257571-Promoter Regions, Genetic,
pubmed-meshheading:3257571-Protein Binding,
pubmed-meshheading:3257571-Sequence Homology, Nucleic Acid,
pubmed-meshheading:3257571-Tetradecanoylphorbol Acetate
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pubmed:year |
1988
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pubmed:articleTitle |
Nuclear proteins interacting with the promoter region of the human granulocyte/macrophage colony-stimulating factor gene.
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pubmed:affiliation |
Division of Human Immunology, Institute of Medical and Veterinary Science, Adelaide, South Australia.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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