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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1989-6-2
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pubmed:abstractText |
D-Lactate in biological samples was converted into the hydrazone of pyruvate in the presence of D-lactate dehydrogenase, an NADH-reoxidation system using diaphorase, DL-6,8-thioctamide and hydrazine. The hydrazone was converted into 2-methylquinoxanol by o-phenylenediamine in hydrochloric acid, and then the quinoxanol was determined by high-performance liquid chromatography with fluorescence detection. The calibration curve of D-lactate was linear up to at least 60 nmol/ml, and the determination limit was 600 fmol. Using this method, D-lactate was determined in biological samples.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9673
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
431
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
239-47
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3243781-Adult,
pubmed-meshheading:3243781-Animals,
pubmed-meshheading:3243781-Chromatography, High Pressure Liquid,
pubmed-meshheading:3243781-Diabetes Mellitus, Experimental,
pubmed-meshheading:3243781-Humans,
pubmed-meshheading:3243781-Hydrazines,
pubmed-meshheading:3243781-Lactates,
pubmed-meshheading:3243781-Lactic Acid,
pubmed-meshheading:3243781-Male,
pubmed-meshheading:3243781-Pyruvates,
pubmed-meshheading:3243781-Rats,
pubmed-meshheading:3243781-Rats, Inbred Strains,
pubmed-meshheading:3243781-Spectrophotometry, Ultraviolet,
pubmed-meshheading:3243781-Yogurt
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pubmed:year |
1988
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pubmed:articleTitle |
Sensitive determination of D-lactic acid in biological samples by high-performance liquid chromatography.
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pubmed:affiliation |
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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