Linked Life Data

3229283

Source:http://linkedlifedata.com/resource/pubmed/id/3229283

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1989-4-13
pubmed:databankReference
pubmed:abstractText
A cDNA library derived from poly(A)+RNA of bull seminal vesicle tissue was screened with a synthetic DNA probe specific for the major protein of bull semen. A positive clone pMP17, containing a 680-bp insert, was sequenced. In combination with primer-extension sequencing of poly(A)+RNA, a DNA sequence of 700 bp was determined. This DNA encodes a reading frame for 134 amino acids, starting with an ATG and terminated by a TAG codon. The first 25 amino acids constitute a signal peptide segment followed by 109 amino acids with the known sequence of the major protein. The initiation methionine occurs within the sequence CTACCATGG, which is highly homologous to a putative control signal for translational efficiency of mammalian mRNAs. The DNA sequence comprises a 3' untranslated region of 276 bp and the polyadenylation signal AATAAA, 13 bp upstream from a tract of A residues. Northern analysis indicated the presence of a 750-bp mRNA species in poly(A)+RNA of seminal vesicle tissue. According to Southern analysis, one gene appears to specify the major protein of bull semen.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0198-0238
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
595-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Cloning and sequence analysis of a cDNA from seminal vesicle tissue encoding the precursor of the major protein of bull semen.
pubmed:affiliation
Max-Planck-Institut für Biophysikalische Chemie, Abt. Molekulare Biologie, Göttingen, W. Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't