Linked Life Data

3215512

Source:http://linkedlifedata.com/resource/pubmed/id/3215512

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12A
pubmed:dateCreated
1989-3-6
pubmed:abstractText
We have investigated the transcriptional regulation of the Antennapedia P2 (Antp P2) promoter using nuclear extracts prepared from Drosophila embryos. Transcriptional analysis of deletion mutants reveals the presence of multiple cis-regulatory elements located both upstream and downstream of the start site. One of the elements appears to mediate negative regulation, since deletion of this element results in higher levels of transcription. Several factors that interact with these control elements have been detected and isolated. One DNA-binding protein, Drosophila Transcription Factor-1 (DTF-1), specifically recognizes the consensus sequence GCAACAT/CG/C that is reiterated four times within an upstream activating element. DTF-1 was purified by sequence-specific DNA affinity chromatography and identified as a doublet of approximately 50 kD. Purified DTF-1 enhances transcription from the Antp P2 promoter 7- to 15-fold in a binding site-dependent manner.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0890-9369
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1615-26
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
In vitro analysis of the Antennapedia P2 promoter: identification of a new Drosophila transcription factor.
pubmed:affiliation
Howard Hughes Medical Institute, Department of Biochemistry, University of California, Berkeley 94720.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't