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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1989-2-7
|
| pubmed:abstractText |
[D-Ala1]peptide T amide is a metabolically stable and more potent analogue of peptide T, a proposed inhibitor of human immunodeficiency viral infectivity of human T-cell lymphocytes. The peptide was synthesized by solid-phase methods to provide amounts of several grams. The product was purified by chromatography on a 25 cm x 2 in. column of DuPont Zorbax Pro-10 C8 (10 micron) packing. Sample loads of 100-450 mg were chromatographed isocratically in 0.1% trifluoroacetic acid and 5% acetonitrile at a flow-rate of 110 ml/min. Under these conditions, the pure peptide fraction was eluted reproducibly between 15 and 22 min. After solvent removal and lyophilization, the recovery of pure peptide was 50% by weight.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0021-9673
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
444
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
345-8
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading | |
| pubmed:year |
1988
|
| pubmed:articleTitle |
Reversed-phase preparative chromatography of [D-Ala1]-peptide T amide.
|
| pubmed:affiliation |
Peptide Technologies Corporation, Trinity College, Washington, DC 20017.
|
| pubmed:publicationType |
Journal Article
|