Linked Life Data

3172826

Source:http://linkedlifedata.com/resource/pubmed/id/3172826

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-11-17
pubmed:abstractText
We describe a method for two-dimensional mapping of tissue sections that makes use of a drawing tube, microscope stage encoders and a microcomputer. The drawing tube views the graphics monitor and superimposes the image of the screen cursor and on-screen menus on the specimen image. Thus, the position of every landmark in each microscopic field can be mapped without stage movement while directly viewing the specimen through the microscope. A mouse is used for data entry and program control. Fields mapped in this way are then assembled into a complete map, which can include line drawings as well as up to 20 landmark types. The coordinate values of all landmarks mapped are stored and remain accessible for editing and analysis. High resolution plots are produced. Specialized functions include grain counting, area and perimeter calculations as well as a perimeter limiter that predefines the area to be mapped. The system uses general purpose hardware that is widely available. Many hitherto time-consuming tasks, such as detailed mapping of cell positions, regions of immunocytochemical staining, degenerating fibers, neuronal connections or any other anatomical feature, can be done in a fraction of the time and effort previously involved. These labor savings can be realized while maintaining the highest resolution and enabling statistical analysis since the data are already in digital form.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0165-0270
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
165-73
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Simple microcomputer system for mapping tissue sections with the light microscope.
pubmed:affiliation
Rockefeller University, New York, NY 10021.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.