rdf:type |
|
lifeskim:mentions |
umls-concept:C0016263,
umls-concept:C0040300,
umls-concept:C0079720,
umls-concept:C0205155,
umls-concept:C0680844,
umls-concept:C0681916,
umls-concept:C0750572,
umls-concept:C1304884,
umls-concept:C1522472,
umls-concept:C1551341,
umls-concept:C1552858,
umls-concept:C1552923,
umls-concept:C1552924,
umls-concept:C1705191,
umls-concept:C1707455,
umls-concept:C1709793
|
pubmed:issue |
4
|
pubmed:dateCreated |
1987-4-17
|
pubmed:abstractText |
A quantitation method for lymphocyte subsets in immunoperoxidase-stained frozen tissue sections was compared with flow cytometry in 23 cases of non-Hodgkin's lymphoma. Close correlations were obtained, demonstrating the accuracy of the technic. Weak intensity of fluorescence and fragility of the tumor cells during the fluorescence-activated cell sorter (FACS) analyses were the most likely explanations for a number of the discrepancies observed. The tissue quantitation method was precise, particularly at low values, where it was better than the FACS. A simpler and faster estimation method employing categories within 10 percentage units was also tested in this study; this method correlated as well with the FACS as the quantitation method and gave the best interobserver correlations.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
AIM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0002-9173
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
87
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
470-7
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:3103419-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:3103419-Antigens, Surface,
pubmed-meshheading:3103419-B-Lymphocytes,
pubmed-meshheading:3103419-Cell Count,
pubmed-meshheading:3103419-Cell Separation,
pubmed-meshheading:3103419-Flow Cytometry,
pubmed-meshheading:3103419-Frozen Sections,
pubmed-meshheading:3103419-Humans,
pubmed-meshheading:3103419-Immunoenzyme Techniques,
pubmed-meshheading:3103419-Lymphocytes,
pubmed-meshheading:3103419-Lymphoma, Non-Hodgkin,
pubmed-meshheading:3103419-Retrospective Studies,
pubmed-meshheading:3103419-T-Lymphocytes
|
pubmed:year |
1987
|
pubmed:articleTitle |
Quantitation and estimation of lymphocyte subsets in tissue sections. Comparison with flow cytometry.
|
pubmed:publicationType |
Journal Article,
Comparative Study
|