Linked Life Data

3099082

Source:http://linkedlifedata.com/resource/pubmed/id/3099082

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1987-2-12
pubmed:abstractText
Double immunoenzymatic method for sequential staining with two different monoclonal murine antibodies and two different enzymes was shown to be useful in defining hematopoietic cell subpopulations in human tissues and blood. The method allows for the identification, localization, and enumeration in the same section of distinct cell populations. Air-dried smears of cell mixtures can be stained. The optimal sequence of enzymes/substrates was: horseradish peroxidase/3-amino-9-ethylcarbazole followed by the alkaline phosphatase-anti-alkaline phosphatase complex/naphthol AS-MX phosphate. Red-and blue-colored reaction products are easy to view in a light microscope. Combinations of two different mouse monoclonal antibodies or of a mouse monoclonal antibody and polyclonal antiserum made in rabbits or goats can be sequentially applied to the same section or smear thus facilitating a definition of the distribution of two cell populations reactive with these antibodies. The relative distribution patterns in tissues of cells bearing distinctive antigens are important in studies of cellular differentiation and of human pathogenetic processes including neoplasia and transplant rejection.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0023-6837
pubmed:author
pubmed:issnType
Print
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
114-9
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Double immunoenzyme staining method for analysis of tissue and blood lymphocyte subsets with monoclonal antibodies.
pubmed:publicationType
Journal Article