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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
1988-10-18
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pubmed:abstractText |
DNAs from human pancreatic adenocarcinomas were analyzed for the presence of mutations in codons 12, 13 and 61 of the NRAS, KRAS and HRAS gene. Formalin-fixed and paraffin-embedded tissue was used directly in an in vitro amplification reaction to expand the relevant RAS sequences. The mutations were detected by selective hybridization using mutation-specific synthetic oligonucleotides. In 28 of the 30 patients we found a mutation in codon 12 of the KRAS gene. This result confirms the findings of Almoguera et al. [Cell 53 (1988) 549-554] that KRAS mutations occur frequently in adenocarcinomas of the exocrine pancreas. The mutations are predominantly G-T transversions, in contrast to the KRAS mutations in colon tumors which are mainly G-A transitions. Furthermore, in a portion of the tumors the mutation appears to be homozygous.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-1149034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-2438556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-2453289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-2826637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-2833702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-2833817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-3103719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-3106803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-3278322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-3283542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-3315232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-3365678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-3470144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-3582981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-3587348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-3871492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-4350660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-6456057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-6595642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3047672-6974040
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7773-82
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3047672-Adenocarcinoma,
pubmed-meshheading:3047672-Base Sequence,
pubmed-meshheading:3047672-Codon,
pubmed-meshheading:3047672-Colonic Neoplasms,
pubmed-meshheading:3047672-Genes, ras,
pubmed-meshheading:3047672-Humans,
pubmed-meshheading:3047672-Lung Neoplasms,
pubmed-meshheading:3047672-Molecular Sequence Data,
pubmed-meshheading:3047672-Mutagens,
pubmed-meshheading:3047672-Mutation,
pubmed-meshheading:3047672-Pancreatic Neoplasms,
pubmed-meshheading:3047672-RNA, Messenger
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pubmed:year |
1988
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pubmed:articleTitle |
KRAS codon 12 mutations occur very frequently in pancreatic adenocarcinomas.
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pubmed:affiliation |
Department of Pathology, State University of Leiden, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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