Linked Life Data

3041594

Source:http://linkedlifedata.com/resource/pubmed/id/3041594

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4866
pubmed:dateCreated
1988-9-8
pubmed:abstractText
The presence of macrophages is required for the regeneration of many cell types during wound healing. Macrophages have been reported to express a wide range of mitogenic factors and cytokines, but none of these factors has been shown in vivo to sustain all the wound-healing processes. It has been suggested that transforming growth factor-alpha (TGF-alpha) may mediate angiogenesis, epidermal regrowth, and formation of granulation tissue in vivo. Macrophages isolated from a wound site, and not exposed to cell culture conditions, expressed messenger RNA transcripts for TGF-alpha, TGF-beta, platelet-derived growth factor A-chain, and insulin-like growth factor-1. The expression of these transcripts was determined by a novel method for RNA analysis in which low numbers of mouse macrophages were isolated from wound cylinders, their RNA was purified and reverse-transcribed, and the complementary DNA was amplified in a polymerase chain reaction primed with growth factor sequence-specific primers. This single-cell RNA phenotyping procedure is rapid and has the potential for quantification, and mRNA transcripts from a single cell or a few cells can be unambiguously demonstrated, with the simultaneous analysis of several mRNA species. Macrophages from wounds expressed TGF-alpha antigen, and wound fluids contained TGF-alpha. Elicited macrophages in culture also expressed TGF-alpha transcripts and polypeptide in a time-dependent manner after stimulation with modified low-density lipoproteins and lipopolysaccharide endotoxin, which are characteristic of the activators found in injured tissues.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0036-8075
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
241
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
708-12
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:3041594-Animals, pubmed-meshheading:3041594-Cell Line, pubmed-meshheading:3041594-DNA, pubmed-meshheading:3041594-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:3041594-Epidermal Growth Factor, pubmed-meshheading:3041594-Fibroblast Growth Factors, pubmed-meshheading:3041594-Fibroblasts, pubmed-meshheading:3041594-Fluorescent Antibody Technique, pubmed-meshheading:3041594-Growth Substances, pubmed-meshheading:3041594-Insulin-Like Growth Factor I, pubmed-meshheading:3041594-Macrophages, pubmed-meshheading:3041594-Male, pubmed-meshheading:3041594-Mice, pubmed-meshheading:3041594-Nucleic Acid Hybridization, pubmed-meshheading:3041594-Peptide Biosynthesis, pubmed-meshheading:3041594-Peptides, pubmed-meshheading:3041594-Platelet-Derived Growth Factor, pubmed-meshheading:3041594-Protein Biosynthesis, pubmed-meshheading:3041594-RNA, Messenger, pubmed-meshheading:3041594-Rabbits, pubmed-meshheading:3041594-Transcription, Genetic, pubmed-meshheading:3041594-Transforming Growth Factors, pubmed-meshheading:3041594-Wound Healing, pubmed-meshheading:3041594-Wounds and Injuries
pubmed:year
1988
pubmed:articleTitle
Wound macrophages express TGF-alpha and other growth factors in vivo: analysis by mRNA phenotyping.
pubmed:affiliation
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't