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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1988-8-31
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pubmed:abstractText |
The long-wavelength ultraviolet (lambda approximately 420 nm) radiation induced reaction between 6-azido-2-methoxy-9-acridinylamines and supercoiled plasmid DNA results in single strand scissions and formation of covalent adducts (ratio approximately 1:10). By treating azidoacridine-photomodified DNA with piperidine at 90 degrees C, additional strand scissions are observed in a complex sequence dependent manner with an overall preference for T greater than or equal to G greater than C much greater than A. The resulting DNA fragments migrate as 5'-phosphates in polyacrylamide gels. Photofootprinting of the binding site of RNA-polymerase on promoter DNA is demonstrated with an azido-9-acridinylamino-octamethylene-9-aminoacridine. Similar experiments using 9-amino-6-azido-2-methoxyacridine indicate that this reagent recognizes changes in the DNA conformation induced by RNA polymerase binding, in relation to open complex formation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-1101952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-16453417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-204861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-212715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-231106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-2411210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-2421408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-2831963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-2836803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-3024713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-3308887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-3322397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-3519983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-3526052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-3543694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-3651386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-3754608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-3896305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-3978094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-4073511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-4092055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-6088528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-6225070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-6497860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-6728031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-673863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3041368-7060576
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5755-70
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
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pubmed:year |
1988
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pubmed:articleTitle |
Photocleavage of DNA and photofootprinting of E. coli RNA polymerase bound to promoter DNA by azido-9-acridinylamines.
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pubmed:affiliation |
Department of Biochemistry B, Panum Institute, University of Copenhagen, Denmark.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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