Linked Life Data

3038794

Source:http://linkedlifedata.com/resource/pubmed/id/3038794

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1987-9-24
pubmed:abstractText
A simple procedure is described for eliminating non-specific staining with avidin-peroxidase conjugates. Murine ovaries were embedded in either paraffin wax or epoxy resin and, after blocking endogenous peroxidase activity, were treated with 10 micrograms/ml biotinylated Pisum sativum agglutinin. Avidin-peroxidase conjugates (5 micrograms/ml), diluted in standard 0.05 M tris-buffered saline, pH 7.6, containing 0.139 M NaCl, produced considerable background coloration and intense mast cell staining in controls without the lectin. This background diminished as the ionic strength of the buffer was raised. At 0.125 M Tris-buffered saline (containing 0.347 M NaCl) the background was completely unstained, with elimination of all binding to mast cells and only minimal loss of specific lectin binding.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0018-2214
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
264-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Elimination of the non-specific binding of avidin to tissue sections.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't