Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-2-23
pubmed:databankReference
pubmed:abstractText
Different forms of glucose-6-phosphate dehydrogenase (G-6-PD) have been described in different tissues. Moreover, the directly determined amino acid sequence amino end of the red cell enzyme does not exactly match the sequence deduced from cDNA isolated from HeLa cells or lymphoblasts. We have therefore investigated the sequence of cDNA from sperm, granulocytes, reticulocytes, brain, placenta, liver, lymphoblastoid cells, and cultured fibroblasts. A novel human cDNA, which has extra 138 bases coding 46 amino acids, was isolated from a lymphoblastoid cell library. Sequencing of genomic DNA amplified by the polymerase chain reaction (PCR) revealed that the extra sequence was derived from the 3'-end of intron 7 by alternative splicing. This longer form of mRNA was also detected in sperm and granulocytes. Sequence analysis using PCR-amplified cDNA revealed that the 5'-end of the coding sequence of G6PD mRNA in reticulocytes is identical to those in other tissues.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-2428611, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-265521, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-2836867, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-2876518, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-2996985, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-2999980, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-3012556, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-3304142, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-3352512, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-3393536, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-3446582, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-3515319, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-3627274, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-3657865, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-3951988, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-4467910, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-4504350, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-518835, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-6407511, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-7063411, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-7106784, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-883990, http://linkedlifedata.com/resource/pubmed/commentcorrection/2910917-956688
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9738
pubmed:author
pubmed:issnType
Print
pubmed:volume
83
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
343-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Alternative splicing of human glucose-6-phosphate dehydrogenase messenger RNA in different tissues.
pubmed:affiliation
Department of Basic and Clinical Research, Research Institute of Scripps Clinic, La Jolla, California 92037.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.