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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1978-11-29
|
| pubmed:abstractText |
The relationship between the subcellular distribution of guanylate cyclase and tissue guanosine-3',5'-cyclic monophosphate (cGMP) levels was investigated in rat testes after surgically induced unilateral cryptorchidism. Placement of one of a testis pair in the abdominal cavity results in loss of testicular weight and function in the abdominal testis whereas the remaining scrotal testis appears to be functionally normal. Within 5 days after surgery, tissue cGMP levels were increased by twofold in the abdominal testis. A fourfold elevation was noted from 10 to 30 days after surgery. Whereas the homogenate guanylate cyclase activity was only slightly elevated 10 and 20 days postoperatively, a 200% increase in the soluble guanylate cyclase activity was seen at 5 days. Between 10 and 30 days, the rise in activity was >250% (P < 0.01). An increase in soluble guanylate cyclase activity was noted when the data were expressed as per milligram protein, per milligram DNA or per whole testis. Conversely, particulate guanylate cyclase activity was reduced by 40% in the cryptorchid testis. Kinetic analysis of the soluble enzyme prepared from abdominal and scrotal testes yielded linear Line-weaver-Burke plots for both enzyme preparations with an identical K(m) for guanosine triphosphate, but a three-fold higher maximal velocity for the abdominal enzyme. When the soluble guanylate cyclases from both testes were mixed and assayed together, the activities were additive rather than exhibiting synergism or inhibition. These experiments indicate that the altered V(max) is not due to a transferable activator or inhibitor.An immunocytochemical technique was used to assess the cell type in which the alterations in cGMP metabolism occurred. Comparison of the scrotal and abdominal testes revealed that the abdominal testis exhibited enhanced cGMP immunofluorescence within the cells lining the inner aspect of the seminiferous tubule as well as tubular elements and interstitial cells. Thus, it is inferred that the correlated changes in soluble guanylate cyclase activity and cGMP levels occur in several of the cell types that remain viable within the cryptorchid testis.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-10967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-12160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-13139144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-13273400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-13293190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-14150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-14691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-14872818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-184122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-188313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-20041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-234425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-236425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-239404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-241938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-4099867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-4150323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-4153733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-4334492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-4359494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-5471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-6469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-66682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/29053-9403
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0021-9738
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
62
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
568-76
|
| pubmed:dateRevised |
2009-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:29053-Animals,
pubmed-meshheading:29053-Cryptorchidism,
pubmed-meshheading:29053-Cyclic GMP,
pubmed-meshheading:29053-Guanylate Cyclase,
pubmed-meshheading:29053-Kinetics,
pubmed-meshheading:29053-Male,
pubmed-meshheading:29053-Rats,
pubmed-meshheading:29053-Solubility,
pubmed-meshheading:29053-Testis
|
| pubmed:year |
1978
|
| pubmed:articleTitle |
Tissue guanosine-3',5'-cyclic monophosphate levels and soluble guanylate cyclase activity: a positive correlation during unilateral cryptorchidism in the rat testis.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|