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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14A
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pubmed:dateCreated |
1988-9-8
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pubmed:abstractText |
Human cyclin/PCNA (proliferating cell nuclear antigen) is structurally, functionally, and immunologically homologous to the calf thymus auxiliary protein for DNA polymerase delta. This auxiliary protein has been investigated as a stimulatory factor for the nuclear DNA polymerases from S. cerevisiae. Calf cyclin/PCNA enhances by more than ten-fold the ability of DNA polymerase III to replicate templates with high template/primer ratios, e.g. poly(dA).oligo(dT) (40:1). The degree of stimulation increases with the template/primer ratio. At a high template/primer ratio, i.e. low primer density, cyclin/PCNA greatly increases processive DNA synthesis by DNA polymerase III. At low template/primer ratios (e.g. poly(dA).oligo(dT) (2.5:1), where addition of cyclin/PCNA only minimally increases the processivity of DNA polymerase III, a several-fold stimulation of total DNA synthesis is still observed. This indicates that cyclin/PCNA may also increase productive binding of DNA polymerase III to the template-primer and stabilize the template-primer-polymerase complex. The activity of yeast DNA polymerases I and II is not affected by addition of cyclin/PCNA. These results strengthen the hypothesis that yeast DNA polymerase III is functionally analogous to the mammalian DNA polymerase delta.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-102692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-2419706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-2869964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-2874992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-2882422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-2882423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-2882424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-2882507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-2884104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-2885320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-2886367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-2891114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-3121622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-320092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-321447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-3275661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-3745189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-4615905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-6145097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-6426510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-6892640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899883-6990186
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6297-307
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2899883-Animals,
pubmed-meshheading:2899883-Cattle,
pubmed-meshheading:2899883-DNA Polymerase III,
pubmed-meshheading:2899883-DNA Replication,
pubmed-meshheading:2899883-DNA-Directed DNA Polymerase,
pubmed-meshheading:2899883-Kinetics,
pubmed-meshheading:2899883-Magnesium,
pubmed-meshheading:2899883-Nuclear Proteins,
pubmed-meshheading:2899883-Proliferating Cell Nuclear Antigen,
pubmed-meshheading:2899883-Saccharomyces cerevisiae,
pubmed-meshheading:2899883-Substrate Specificity,
pubmed-meshheading:2899883-Templates, Genetic
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pubmed:year |
1988
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pubmed:articleTitle |
Mammalian cyclin/PCNA (DNA polymerase delta auxiliary protein) stimulates processive DNA synthesis by yeast DNA polymerase III.
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pubmed:affiliation |
Department of Biological Chemistry, Washington University School of Medicine, St. Louis, MO 63110.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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