rdf:type |
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lifeskim:mentions |
|
pubmed:issue |
3
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pubmed:dateCreated |
1988-6-14
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pubmed:databankReference |
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pubmed:abstractText |
The genome of the avian erythroblastosis virus temperature sensitive mutant ts34 was cloned from a cell line that was shown to contain a single integrated copy of the virus. The mutation was localized to the v-erbB gene by making chimeric viruses between the mutant genome and that of wildtype. Sequencing of the mutant v-erbB gene revealed a single amino acid change of a histidine to an aspartate residue at a position equivalent to amino-acid 826 of the human epidermal growth factor receptor. Interestingly this is the identical mutation to that recently reported for another temperature sensitive mutant ts167, indicating that this may be a hot spot for mutations in the v-erbB gene that give rise to ts transformation mutants. In addition, the different biological phenotypes of ts34 and ts167 are suggested to be due to an additional mutation in v-erbA in ts167.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0890-6467
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
1
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
265-78
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2897102-Alpharetrovirus,
pubmed-meshheading:2897102-Amino Acid Sequence,
pubmed-meshheading:2897102-Avian leukosis virus,
pubmed-meshheading:2897102-Base Sequence,
pubmed-meshheading:2897102-Cell Line,
pubmed-meshheading:2897102-Cell Transformation, Neoplastic,
pubmed-meshheading:2897102-Cloning, Molecular,
pubmed-meshheading:2897102-DNA, Viral,
pubmed-meshheading:2897102-DNA Restriction Enzymes,
pubmed-meshheading:2897102-Genes, Viral,
pubmed-meshheading:2897102-Molecular Sequence Data,
pubmed-meshheading:2897102-Mutation,
pubmed-meshheading:2897102-Oncogene Proteins, Viral,
pubmed-meshheading:2897102-Oncogene Proteins v-erbB,
pubmed-meshheading:2897102-Plasmids,
pubmed-meshheading:2897102-Temperature,
pubmed-meshheading:2897102-Viral Proteins
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pubmed:year |
1987
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pubmed:articleTitle |
Common site of mutation in the erbB gene of avian erythroblastosis virus mutants that are temperature sensitive for transformation.
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pubmed:affiliation |
Imperial Cancer Research Fund Laboratories, St Bartholomews Hospital, London.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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