Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
1987-12-23
pubmed:databankReference
pubmed:abstractText
A 2.2-kilobase clone comprising a major portion of the coding sequence of the Na+,K+-ATPase alpha subunit was cloned from human placenta and its sequence was identical to that encoding the alpha subunit of human kidney and HeLa cells. Transfer blot analysis of the mRNA products of the Na+,K+-ATPase gene from various human tissues and cell lines revealed only one band (approximately 4.7 kilobases) under low and high stringency washing conditions. The levels of expression in the tissues were intestine greater than placenta greater than liver greater than pancreas, and in the cell lines the levels were human erythroleukemia greater than butyrate-induced colon greater than colon greater than brain greater than HeLa cells. mRNA was undetectable in reticulocytes, consistent with our failure to detect positive clones in a size-selected (greater than 2 kilobases) lambda gt11 reticulocyte cDNA library. DNA analysis revealed a polymorphic EcoRI band and chromosome localization by flow sorting and in situ hybridization showed that the alpha subunit is on the short arm (band p11-p13) of chromosome 1.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-1262041, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-2430951, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-2859121, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-2993903, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-2993905, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-2994074, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-3005037, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-3008098, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-3025616, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-3028470, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-3035563, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-3462737, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-3470951, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-3948148, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-4377446, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-4504350, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-518835, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-6194404, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-6276651, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-6289898, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-6290987, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-6310323, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-6587566, http://linkedlifedata.com/resource/pubmed/commentcorrection/2891135-911786
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7901-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Human placental Na+,K+-ATPase alpha subunit: cDNA cloning, tissue expression, DNA polymorphism, and chromosomal localization.
pubmed:affiliation
Howard Hughes Medical Institute University of California, San Francisco 94143.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't