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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
1987-8-20
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pubmed:abstractText |
A protein with biological activities similar to parathyroid hormone (PTH) has been purified from serum-free culture medium obtained from a human lung cancer cell line (BEN). A major protein band of 18 kDa was obtained on NaDodSO4/polyacrylamide gels, with faint bands at 35 kDa and 67 kDa. Biological activity was associated only with the 18-kDa band. Amino acid sequence analysis of the material purified by HPLC revealed that 8 of the 16 residues were identical with those of human PTH. Antibody raised to a corresponding synthetic peptide recognized the PTH-related material but showed less than 1% cross-reactivity with human PTH amino-terminal peptides. BEN cells contained PTH DNA, but not PTH messenger RNA, indicating involvement of another gene. The purified PTH-related protein had a specific biological activity approximately equal to 6 times greater than that of bovine PTH(1-34). The PTH-related protein may have a role in the syndrome of humoral hypercalcemia of malignancy.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-1130679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-1233082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-3005460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-3745199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-3859875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-3948772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-3967626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-4059056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-4712989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-4831319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-6220408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-6244091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-6253785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-6270184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-6298282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-6298628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-6298791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-6321505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-6328302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-6499773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-6685137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-6755168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-6866067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2885845-708370
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Hormones, Ectopic,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PTHLH protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Parathyroid Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Parathyroid Hormone-Related Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Poly A,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Teriparatide
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
5048-52
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2885845-Amino Acid Sequence,
pubmed-meshheading:2885845-Carcinoma, Squamous Cell,
pubmed-meshheading:2885845-Cell Line,
pubmed-meshheading:2885845-Cross Reactions,
pubmed-meshheading:2885845-Cyclic AMP,
pubmed-meshheading:2885845-DNA, Neoplasm,
pubmed-meshheading:2885845-Hormones, Ectopic,
pubmed-meshheading:2885845-Humans,
pubmed-meshheading:2885845-Hypercalcemia,
pubmed-meshheading:2885845-Lung Neoplasms,
pubmed-meshheading:2885845-Neoplasm Proteins,
pubmed-meshheading:2885845-Paraneoplastic Endocrine Syndromes,
pubmed-meshheading:2885845-Parathyroid Hormone,
pubmed-meshheading:2885845-Parathyroid Hormone-Related Protein,
pubmed-meshheading:2885845-Peptide Fragments,
pubmed-meshheading:2885845-Peptides,
pubmed-meshheading:2885845-Poly A,
pubmed-meshheading:2885845-RNA, Messenger,
pubmed-meshheading:2885845-RNA, Neoplasm,
pubmed-meshheading:2885845-Teriparatide
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pubmed:year |
1987
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pubmed:articleTitle |
Parathyroid hormone-related protein purified from a human lung cancer cell line.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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