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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1988-4-14
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pubmed:abstractText |
Previous work has established that the v-erbA oncogene inhibits the temperature-induced differentiation of chick erythroblasts transformed with temperature-sensitive oncogene mutants. Here we demonstrate that v-erbA in differentiating erythroblasts specifically arrests expression of the erythrocyte anion transporter (band 3) gene at the transcriptional level. The v-erbA-induced differentiation block can be overcome by inducing cells to differentiate at alkaline pH. Under these conditions, which possibly impair biological activity of v-erbA, the maturing cells now express the anion transporter gene at high levels. However, its transcription is specifically and rapidly suppressed if v-erbA activity is restored by culturing the cells at neutral pH. Similar but less pronounced inhibition of gene expression by v-erbA was observed for the delta-amino-levulinic acid synthase gene. Additional evidence obtained with an inhibitor of band 3 activity suggests that the v-erbA-induced inhibition of band 3 gene expression is at least partly responsible for the differentiation block caused by this oncogene.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
52
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
107-19
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2830979-5-Aminolevulinate Synthetase,
pubmed-meshheading:2830979-Alpharetrovirus,
pubmed-meshheading:2830979-Animals,
pubmed-meshheading:2830979-Anion Transport Proteins,
pubmed-meshheading:2830979-Avian leukosis virus,
pubmed-meshheading:2830979-Carrier Proteins,
pubmed-meshheading:2830979-Cell Transformation, Viral,
pubmed-meshheading:2830979-Cells, Cultured,
pubmed-meshheading:2830979-Chick Embryo,
pubmed-meshheading:2830979-Chickens,
pubmed-meshheading:2830979-Clone Cells,
pubmed-meshheading:2830979-Culture Media,
pubmed-meshheading:2830979-Cytoskeletal Proteins,
pubmed-meshheading:2830979-Erythroblasts,
pubmed-meshheading:2830979-Fibroblasts,
pubmed-meshheading:2830979-Fluorescent Antibody Technique,
pubmed-meshheading:2830979-Gene Expression Regulation,
pubmed-meshheading:2830979-Globins,
pubmed-meshheading:2830979-Hemoglobins,
pubmed-meshheading:2830979-Hydrogen-Ion Concentration,
pubmed-meshheading:2830979-Immunoassay,
pubmed-meshheading:2830979-Mutation,
pubmed-meshheading:2830979-Oncogenes,
pubmed-meshheading:2830979-RNA, Messenger,
pubmed-meshheading:2830979-Spectrin,
pubmed-meshheading:2830979-Suppression, Genetic,
pubmed-meshheading:2830979-Temperature,
pubmed-meshheading:2830979-Transcription, Genetic
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pubmed:year |
1988
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pubmed:articleTitle |
v-erbA specifically suppresses transcription of the avian erythrocyte anion transporter (band 3) gene.
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pubmed:affiliation |
European Molecular Biology Laboratory Differentiation Program, Heidelberg, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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