Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
|
pubmed:dateCreated |
1987-12-31
|
pubmed:abstractText |
We report the isolation and analysis of genomic clones comprising the entire gene coding for the human muscle carbonic anhydrase, CAIII. The gene spans 10.3 kb and has a seven-exon/six-intron structure. A noncanonical TATA box, a CCAAT motif, and two CCGCCC elements are present in the sequences upstream of exon 1. Although the expression of CAIII shows strict tissue specificity, the gene exhibits a number of features normally associated with housekeeping enzymes. For example, there is 48% homology with a 25-bp consensus sequence between the TATA box and the cap site and there is a CpG-rich island spanning a 469-bp sequence near to the origin of transcription. Methylation studies suggest that some CCGG sites clustered in the CpG-rich island are undermethylated in DNA from fetal and adult muscle and in other tissues irrespective of CAIII expression. In contrast, several nonclustered CCGG sites show a methylation pattern that correlates with gene expression. However DNA from differentiated type II adult muscle fibers is undermethylated at these sites even though CAIII is not expressed.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Aug
|
pubmed:issn |
0890-9369
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
1
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
594-602
|
pubmed:dateRevised |
2008-11-21
|
pubmed:meshHeading |
pubmed-meshheading:2824285-Adult,
pubmed-meshheading:2824285-Amino Acid Sequence,
pubmed-meshheading:2824285-Base Sequence,
pubmed-meshheading:2824285-Carbonic Anhydrases,
pubmed-meshheading:2824285-DNA Restriction Enzymes,
pubmed-meshheading:2824285-Exons,
pubmed-meshheading:2824285-Fetus,
pubmed-meshheading:2824285-Genes,
pubmed-meshheading:2824285-Humans,
pubmed-meshheading:2824285-Introns,
pubmed-meshheading:2824285-Methylation,
pubmed-meshheading:2824285-Molecular Sequence Data,
pubmed-meshheading:2824285-Muscles,
pubmed-meshheading:2824285-Promoter Regions, Genetic
|
pubmed:year |
1987
|
pubmed:articleTitle |
Human muscle carbonic anhydrase: gene structure and DNA methylation patterns in fetal and adult tissues.
|
pubmed:affiliation |
Human Biochemical Genetics Unit, Galton Laboratory, University College London, UK.
|
pubmed:publicationType |
Journal Article,
Comparative Study
|